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[KO]Catalase Rabbit mAb

Western blot analysis of Catalase in HeLa(WT) and Catalase-Knockout HeLa(KO) using Catalase antibody.
Western blot analysis of Catalase in K562, Hela lysates using Catalase antibody.
Immunocytochemistry analysis of Catalase(green) in Hela using Catalase antibody,and DAPI(blue)
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Catalase antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F
Primary Accession	P04040
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal Antibody
Isotype	IgG
Conjugate	Unconjugated
Purification	Affinity Purified
Calculated MW	59756 Da

Additional Information
Gene ID	847
Other Names	CAT
Dilution	WB~~1:1000-1:5000 IHC-P~~1:50~200 IHC-F~~N/A
Format	1xPBS(pH 7.4), 150mM NaCl, 50% Glycerol, 0.02% Sodium azide and 0.05% BSA
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	CAT
Function	Catalyzes the degradation of hydrogen peroxide (H(2)O(2)) generated by peroxisomal oxidases to water and oxygen, thereby protecting cells from the toxic effects of hydrogen peroxide (PubMed:7882369). Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells (PubMed:7882369).
Cellular Location	Peroxisome matrix

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen. Research studies show that overexpression of this antioxidant enzyme increases the ability of pancreatic β-cells to scavenge reactive oxygen species (ROS), thereby protecting pancreatic β-cells from oxidative stress.

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¥ 1,250.00

Cat# AP75200

Size:

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100 µl

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¥ 2,050.00

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[KO]Catalase Rabbit mAb

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